Efeito de concentrações de sacarose sobre o crescimento in vitro e a aclimatização da bromélia nativa Vriesea inflata (Wawra) Wawra

Biotemas

Victória Carvalho¹  Camila Freitas¹  Catarina Carvalho Nievola¹ 

[1] Instituto de Botânica SMA/SP

关键词: Bromeliaceae;    Ex vitro;    Fonte de carbono;    Micropropagação;    Ornamental;   

DOI  :  

学科分类：生物科学（综合）

来源: Universidade Federal de Santa Catarina * Centro de Ciencias Biologicas

【 摘 要 】

http://dx.doi.org/10.5007/2175-7925.2015v28n3p37Using sucrose is crucial forin vitroplant growth, due to decreased photosynthesis under this condition. However, ornamental epiphytic species, which have slow growth, have shown better development in nutrient media with low concentrations of this carbohydrate.Vriesea inflata(Wawra) Wawra is an epiphytic bromeliad native to the threatened Atlantic Rain Forest and it has a high ornamental value. This study aimed to evaluate the effects of various sucrose concentrations in the culture medium on in vitro growth and subsequentex vitroacclimatization. Plants were cultured in vitro in basal media without carbohydrates; and in media containing 1.0; 1.5; 3.0; 4.5; and 6.0% (w/v) of sucrose for 60 days. After, plants were acclimatized for 90 days. Plants cultured in sucrose concentrations over 3.0% had the smallest development after in vitro culture and acclimatization. Nevertheless, those cultured at lower concentrations had the highest in vitro root growth, and they generated larger plants after acclimatization. On average, 1.7% sucrose was defined as appropriate to cultivateV. inflata in vitro , in order to obtain more vigorous plants during acclimatization.

【 授权许可】

Unknown