Apoptosis Induction of POS Canine Osteosarcoma cells by Vitamin D and Retinoids

Journal of Veterinary Medical Science

Edward F. BARROGA¹  Masahiro OKUMURA¹  Toru FUJINAGA¹  Tsuyoshi KADOSAWA¹  Kazushi ASANO¹ 

[1] Laboratory of Veterinary Surgery, Department of Veterinary Clinical Sciences, Graduate School of Veterinary Medicine, Hokkaido University

关键词: apoptosis;    canine osteosarcoma;    vitamin;   

DOI  :  10.1292/jvms.60.1269

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(19)Cited-By(12)Vitamin D3: 1-α,25(OH) 2D3 (calcitriol), 22-oxa-1,25(OH)2D 3 (OCT), cholecalciferol (vitamin D3), and retinoids: all-trans retinoic acid (ATRA) and 9-cis retinoic acid, induced morphological changes in POS canine osteosarcoma cells into elongated, spindle or fibroblast like-shaped cells, and apoptotic like cell death characterized by cell shrinkage, condensation and margination of the nucleus for all drugs at 10-6M-10 -9M after 72 to 120 hr culture. Apoptosis as shown by DNA laddering was induced at 48 hr by all drugs at 10-6M, 10-7M at 96 hr, 10-8M and 10-9M at 120 hr respectively. These vitamins are suggested to adjunct antineoplastic agents in canine osteosarcoma therapy by induction of apoptosis.

【 授权许可】

Unknown