Characterization of a Neutralizing Monoclonal Antibody against Botulinum ADP-Ribosyltransferase, C3 Exoenzyme

Journal of Veterinary Medical Science

Hidenobu HOSHI¹  Shunji KOZAKI¹  Yoichi KAMATA¹  Hayato CHOKI¹ 

[1] Department of Veterinary Science, College of Agriculture, Osaka Prefecture University

关键词: ADP-ribosylation;    biological effect;    botulinum C3 exoenzyme;    monoclonal antibody;   

DOI  :  10.1292/jvms.64.767

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(22)Cited-By(1)A monoclonal antibody, named C302, was prepared and characterized against botulinum ADP-ribosyltransferase C3 exoenzyme that inactivates RhoA GTP-binding protein, resulting in the neurite outgrowth of human neuroblastoma GOTO cells. C302 bound not to the smaller fragments derived from the protease-treated C3 exoenzyme but to the intact C3 exoenzyme. It seems that the C302 epitope may depend on the three-dimensional structure of C3 exoenzyme molecule. C302 depressed the enzymatic and biological actions of C3 exoenzyme. The dose-dependent depression pattern of C302 on the enzyme activity was similar to that to the biological one. C302 turned the neurite-bearing shape of the C3 exoenzyme-treated GOTO cells into the intact shape. By using of C302 mAb and C3 exoenzyme, the research concerning GTP-binding proteins would be improved.

【 授权许可】

Unknown