Effects of 17β-Estradiol and the Japanese Herbal Medicine Keishi-bukuryo-gan on the Release and Synthesis of Calcitonin Gene-Related Peptide in Ovariectomized Rats

Journal of Pharmacological Sciences

Mitsutoshi Yuzurihara¹  Masamichi Noguchi¹  Yoshio Kase¹  Yasushi Ikarashi¹  Shuichi Takeda¹  Masaki Aburada¹ 

[1] Tsumura Research Institute Medical Evaluation Laboratory

关键词: calcitonin gene-related peptide;    17β-estradiol;    Keishi-bukuryo-gan;    ovariectomized rat;    hot flash;   

DOI  :  10.1254/jphs.93.80

学科分类：药学

来源: Nihon Yakuri Gakkai Henshuubu / Japanese Pharmacological Society

【 摘 要 】

References(25)Cited-By(6)The purpose of this study is to clarify the effects of 17β-estradiol and the Japanese herbal medicine Keishi-bukuryo-gan on the release and synthesis of calcitonin gene-related peptide (CGRP) in ovariectomized (OVX) rats. The effect of ovariectomy on the release or synthesis was evaluated by measuring CGRP concentration in plasma after capsaicin (1.0 mg/kg, i.p.) injection or by measuring CGRP concentration and its mRNA expression in dorsal root ganglia in OVX rats. Ovariectomy attenuated the capsaicin-evoked increase in plasma concentration of CGRP, which was restored by treatment with 17β-estradiol (0.010 mg/kg, s.c.) or Keishi-bukuryo-gan (1,000 mg/kg, p.o.) for 7 days after ovariectomy. However, no significant differences were observed in the CGRP concentration and the mRNA expression of dorsal root ganglia by treating the rats with ovariectomy, 17 β-estradiol, and Keishi-bukuryo-gan. These results suggest not only that estrogen deficiency attenuates CGRP release but also that 17β-estradiol or Keishi-bukuryo-gan normalizes the attenuated release process.

【 授权许可】

Unknown