Direct Assessments of the Antioxidant Effects of the Novel Collagen Peptide on Reactive Oxygen Species Using Electron Spin Resonance Spectroscopy

Journal of Pharmacological Sciences

Fumihiko Yoshino¹  Shunji Hattori²  Keisuke Tanaka²  Yosuke Kawamura¹  Kyo Kobayashi¹  Tomoko Komatsu³  Satoko Wada-Takahashi¹  Masaichi-Chang-Il Lee¹  Chihiro Miyamoto¹  Shun-Suke Takahashi¹  Ayaka Yoshida¹  Yojiro Maehata¹  Masashi Kusubata² 

[1] Division of Pharmacology and ESR Laboratories, Department of Clinical Care Medicine, Kanagawa Dental College, Japan;Nippi Research Institute of Biomatrix, Japan;Division of Dentistry for Special Patients, Department of Clinical Care Medicine, Kanagawa Dental College, Japan

关键词: singlet oxygen;    hydroxyl radical;    photo-aging;    human fibroblast;    ultraviolet ray A (UVA) irradiation;   

DOI  :  10.1254/jphs.10321FP

学科分类：药学

来源: Nihon Yakuri Gakkai Henshuubu / Japanese Pharmacological Society

【 摘 要 】

References(33)Cited-By(3)In the present study, we evaluated the antioxidant effects of a pepsin-treated novel collagen peptide (P-NCP) on reactive oxygen species (ROS) such as hydroxyl radical (HO•), superoxide anion radical (O2•–), and singlet oxygen (1O2), and the effects on cell viability after ultraviolet ray (UV) irradiation of human fibroblasts. We confirmed, using electron spin resonance, that P-NCP directly inhibited HO• and 1O2. Furthermore, addition of P-NCP to fibroblasts inhibited cell death induced by UVA (400 – 315 nm) irradiation in a dose-dependent manner. In addition, the antioxidant effect on 1O2 was observed in the peptide fractions rich in Gly, Pro, Hyp, Glu, Ala, and Arg. We found that Gly, Hyp, Glu, and Ala directly scavenged 1O2. These results indicated that a peptide sequence including Gly, Hyp, Glu, and Ala could play a key role in the antioxidant effects of P-NCP on 1O2. It was suggested that P-NCP can inhibit photo-aging related to ROS owing to its antioxidant effects.

【 授权许可】

Unknown