Cytotoxicity for Porcine Islet Cells by Complement of Six Animal Species

Journal of Veterinary Medical Science

Tomofumi JITSUKAWA²  Hisamune KATO⁴  Pi-Chao WANG⁴  Kazuya EDAMURA¹  Atsuko MATSUNAGA²  Shin ITAKURA¹  Nobuo SASAKI¹  Ryohei NISHIMURA¹  Manabu MOCHIZUKI¹  Hisako OHGAWARA³ 

[1] Laboratory of Veterinary Surgery, Graduate School of Agricultural and Life Sciences, The University of Tokyo;Axia Biosciences, Inc., Shinanomachi Campus-Research Park, Keio University School of Medicine;Medical Research Institute, Tokyo Women's Medical University School of Medicine;Institute of Applied Biochemistry, Tsukuba University

关键词: complement;    cytotoxicity;    porcine islet cell;   

DOI  :  10.1292/jvms.65.1107

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(12)Complement-mediated cytotoxicity for porcine islet cells (PICs) was evaluated using sera of six animal species. Then soluble complement receptor type-1 (sCR1) as an anti-complement agent was added to those sera, and the changes in 50% hemolytic unit of complement serum (CH50) and cytotoxic effect of those sera on PICs were examined. All the sera except for that of pig showed cytotoxicity. However, the extent of toxicity was considerably different between species. In the rat and human serum, sCR1 significantly reduced CH50 and cytotoxicity, however in the dog serum, sCR1 had no suppressive effects. These results may suggest that complement contribute to humoral cytotoxicity for PICs as a main factor, and the compatibility of complement with PICs differs between animal species.

【 授权许可】

Unknown