Spatiotemporal Distribution of Chondroitin Sulfate Proteoglycans in the Developing Mouse Retina and Optic Nerve

Journal of Veterinary Medical Science

Masato UEHARA¹  Yoshinao Z. HOSAKA¹  Safwat Ali Mohamed ALI¹ 

[1] Laboratory of Basic Veterinary Science, United Graduate School of Veterinary Science, Yamaguchi University

关键词: chondroitin sulfate proteoglycan;    development;    optic nerve;    retina;    spatiotemporal distribution;   

DOI  :  10.1292/jvms.10-0201

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(45)Cited-By(2)The aim of the present study was to determine the distribution of chondroitin sulfate proteoglycans in the mouse retina and optic nerve of the prenatal and postnatal mouse by immunohistochemistry. At embryonic day (E) 18, chondroitin-4-sulfate (C4S), chondroitin-6-sulfate (C6S) and biglycan were detected in the retina and optic nerve. However, aggrecan was seen in the retina but not in the optic nerve. At postnatal day (P) 7, aggrecan and biglycan were clearly observed in the optic nerve, inner nuclear layer and ganglion cell layer and diffuse in the outer retina. C4S diffusely distributed in the retina and optic nerve, but C6S was mainly confined to the photoreceptor layer and optic nerve sheath. At P42, biglycan showed diffuse distribution in the retina and optic nerve with intense staining in nerve-fiber rich layers. Aggrecan showed weak staining at the inner plexiform layer with higher density in the outer and inner nuclear layers, outer plexiform layer and ganglion cell layer. Both C4S and C6S were detected in the optic nerve and retina, but C6S showed strong immunostaining in the photoreceptor layer. The distributions of these proteoglycans with respect of time course during development of the retina and optic nerve suggest that they may have unique or overlapping roles in development and maintenance of the retina and optic nerve.

【 授权许可】

Unknown