Extraction of ecdysterone from roots of Brazilian ginseng

Ciência Rural

Maldaner, Joseila²  Nicoloso, Fernando Teixeira²  Cezarotto, Verciane¹  Flores, Rejane²  Giacomelli, Sandro Rogério¹  Brondani, Daniela¹ 

[1] Universidade Regional Integrada, Frederico Westphalen, Brasil;Universidade Federal de Santa Maria, Santa Maria, Brasil

关键词: Pfaffia glomerata;    Pfaffia tuberosa;    ecdysteroids;    phytomedicine;    quality control;   

DOI  :  10.1590/S0103-84782009000400042

学科分类：农业科学（综合）

来源: Universidade Federal de Santa Maria * Centro de Ciencias Rurais

【 摘 要 】

This study aimed at optimizing the extraction method from ecdysterone of Brazilian ginseng. Root samples of two accessions (BRA and JB-UFSM) of P. glomerata were extracted in a Soxhlet with methanol or chloroform for 4h. In the second trial, the extration was conduced in a Soxhlet or ultrasonic using metanol as a solvent. In P. tuberosa, the roots samples were extracted with methanol in a Soxhlet or in ultrasonic. The ecdysterone content was determinated using high efficiency liquid chromatography methods. In both studied species, the highest ecdisterone content was detected from samples extracted in a Soxhlet and using methanol as a solvent. This extration method has been successfully applied for determination of ecdysterone content from roots of Brazilian ginseng, and could be useful for the quality control of drugs and pharmaceutical formulations.

【 授权许可】

CC BY-NC