Inhibition of Phenotypic and Functional Maturation of Dendritic Cells by Manassantin A

Journal of Pharmacological Sciences

Young Kook Kim¹  Youngsoo Kim²  Jong Soon Kang¹  Hong Kyung Lee²  Jee Youn Kim²  Jin Tae Hong²  Sang-Bae Han²  Sukgil Song²  Hwan Mook Kim¹ 

[1] Korea Research Institute of Bioscience and Biotechnology, Korea;College of Pharmacy, Chungbuk National University, Korea

关键词: manassantin A;    dendritic cell;    maturation;    mitogen-activated protein kinase;    NF-κB;   

DOI  :  10.1254/jphs.08299FP

学科分类：药学

来源: Nihon Yakuri Gakkai Henshuubu / Japanese Pharmacological Society

【 摘 要 】

References(37)Cited-By(13)Manassantin A (MSA) inhibits nitric oxide production by macrophages through the inhibition of NF-κB activation, but the effect of MSA on dendritic cells has not been elucidated yet. Here we investigated the inhibitory effects of MSA on immune functions of dendritic cells (DCs). MSA inhibited lipopolysaccharide (LPS)-induced phenotypic maturation of DCs, which was proved by the decreased expression of CD40, CD80, CD86, MHC-I, and MHC-II. MSA also inhibited functional maturation of DCs, that is, decreased the gene expression of IL-12, IL-1β, TNF-α, and IFN-α/β; enhanced antigen capture capacity of DCs; and impaired induction of allogenic T cell activation. As a mechanism of action, MSA inhibited LPS-induced activation of NF-κB, ERK, p38, and JNK, which played pivotal roles in toll-like receptor 4–mediated DC maturation. Collectively, these results suggested that MSA might be used for the treatment of DC-related immune diseases.

【 授权许可】

Unknown