Total Glutathione and Glutathione Reductase in Bovine Erythrocytes and Liver Biopsy

Journal of Veterinary Medical Science

Jun YASUDA³  Mahmoud Rushdi ABD ELLAH³  Masanobu GORYO¹  Saori KOBAYASHI²  Akihiro OISHI³  Keiji OKADA³ 

[1] Department of Disease Control and Environmental Science, Faculty of Agriculture, Iwate University;Department of Clinical Veterinary Science, The United Graduate School of Veterinary Medicine, Gifu University;Veterinary Teaching Hospital, Faculty of Agriculture, Iwate University

关键词: bovine liver biopsy;    glutathione;    glutathione reductase;   

DOI  :  10.1292/jvms.70.861

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(26)Cited-By(2)The goal of the present study was to measure the total glutathione level and glutathione reductase activity in bovine erythrocytes and liver biopsy. Five cows were injected intraperitoneally with DL-ethionine (12.5 mg/kg B.W.), and two control cows were injected with normal saline (0.9% NaCl). Ultrasonography guided liver biopsy, and blood samples were collected at 0, 4, 7 and 10 days after injection. The hepatic total glutathione level was significantly increased on Days 7 (p<0.05) and 10 (p<0.01), and hepatic glutathione reductase activity was significantly increased on Days 4 (p<0.05), 7 (p<0.01) and 10 (p<0.01). There were insignificant changes in the erythrocytic total glutathione level. The present study demonstrated that liver biopsy is a valuable tool for detecting oxidative stress and for diagnosing hepatic dysfunction in cattle from the viewpoint of the status of glutathione and glutathione reductase.

【 授权许可】

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