Endothelin Type B Receptor–Induced Sustained Ca2+ Influx Involves Gq/11/Phospholipase C–Independent, p38 Mitogen-Activated Protein Kinase–Dependent Activation of Na+/H+ Exchanger

Journal of Pharmacological Sciences

Hiroyuki Aoyagi¹  Tadashi Nishiya¹  Hiroshi Asano¹  Ikunobu Muramatsu²  Takahiro Horinouchi¹  Tsunaki Higa¹  Arata Nishimoto¹  Soichi Miwa¹ 

[1] Department of Cellular Pharmacology, Hokkaido University Graduate School of Medicine, Japan;Division of Pharmacology, Department of Biochemistry and Bioinformative Sciences, School of Medicine, University of Fukui, Japan

关键词: endothelin type B receptor;    Na+/H+ exchanger;    p38 mitogen-activated protein kinase;   

DOI  :  10.1254/jphs.10102SC

学科分类：药学

来源: Nihon Yakuri Gakkai Henshuubu / Japanese Pharmacological Society

【 摘 要 】

References(15)Cited-By(4)The mechanism for sustained Ca2+ influx activated by G protein–coupled receptors was examined. In Chinese hamster ovary cells expressing recombinant human endothelin type B receptor (ETBR) and endogenous P2Y receptor (P2Y-R), endothelin-1 elicited a sustained Ca2+ influx depending on Gq/11 protein, phospholipase C (PLC), Na+/H+ exchanger (NHE), and p38 mitogen-activated protein kinase (p38MAPK), whereas P2Y-R–induced sustained Ca2+ influx was negligible. Functional studies showed that NHE activation by ETBR was mediated via p38MAPK but not Gq/11/PLC, while that by P2Y-R involves only Gq/11/PLC/p38MAPK. These results suggest that Gq/11/PLC-independent NHE activation via p38MAPK plays an important role in ETBR- mediated sustained Ca2+ influx.

【 授权许可】

Unknown