Molecular detection of Erwinia psidii in guava plants under greenhouse and field conditions

Ciência Rural

Ferreira, Marisa Álvares da Silva Velloso²  Marques, Abi Soares dos Anjos¹  Blum, Luiz Eduardo Bassay²  Uesugi, Carlos Hidemi²  Silva, Claudênia Ferreira da² 

[1] Embrapa Quarentena Vegetal, Brasília, Brazil;Universidade de Brasília, Brasília, Brazil

关键词: BIO-PCR;    diagnosis;    guava bacterial blight;    PCR;    Psidium guajava;   

DOI  :  10.1590/0103-8478cr20151600

学科分类：农业科学（综合）

来源: Universidade Federal de Santa Maria * Centro de Ciencias Rurais

【 摘 要 】

: Erwinia psidii causes bacterial blight of guava ( Psidium guajava), an important disease of this crop in Brazil. The pathogen affects branches and twigs of guava trees, reducing yield significantly. Bacterial dissemination often occurs through contaminated but asymptomatic propagating plant material. The objectives of this research were to evaluate the use of BIO-PCR and conventional PCR to detect E. psidiiin inoculated guava plants grown in a greenhouse and in symptomatic and asymptomatic trees from guava orchards. Erwinia psidiistrain IBSBF 1576 was inoculated (107CFU mL-1) into young guava shoots and plant tissue was analysed at 0, 5, 10, and 15 days after inoculation. Symptoms were observed after 5 days and all inoculated shoots were PCR positive at all times, by both BIO-PCR and conventional PCR. Under natural infection conditions, 40 samples were tested by BIO-PCR from each of three guava orchards, 20 showing symptoms and 20 asymptomatic. PCR was positive for 58 out of 60 symptomatic samples (96.7%) and for 6.7% of asymptomatic samples, showing that the method can be used to detect the pathogen at early stages of infection. This PCR method may be used as a diagnostic tool to assess bacterial survival, dissemination and disease outbreaks.

【 授权许可】

Unknown