Phenotypic characterization of the bone marrow stem cells used in regenerative cellular therapy

Revista Cubana de Hematología, Inmunología y Hemoterapia

Hernández-Ramírez, Porfirio¹  Palma-Salgado, Lourdes¹  Dorticós-Balea, Elvira¹  Marsán-Suárez, Vianed¹  Baganet-Cobas, Aymara¹  Ballester-Santovenia, José M¹  Macías-Abraham, Consuelo²  Sánchez-Segura, Miriam¹  Socarrás-Ferrer, Bertha B¹  del Valle-Pérez, Lázaro O¹  Jaime-Fagundo, Juan C¹  Lam-Díaz, Rosa M¹ 

[1] Instituto de Hematología e Inmunología, La Habana, Cuba

关键词: Células madre;    células progenitoras;    medicina regenerativa;    células mesenquimales;    células endoteliales;   

DOI  :  

学科分类：生理学与病理学

来源: SciELO

【 摘 要 】

Regenerative medicine is a novel therapeutic method with broad potential for the treatment of various illnesses, based on the use of bone marrow (BM) stem cells, whose phenotypic characterization is limited. The paper deals with the expression of different cell membrane markers in mononuclear BM cells from 14 patients who underwent autologous cell therapy, obtained by medullary puncture and mobilization to peripheral blood, with the purpose of characterizing the different types of cells present in that heterogeneous cellular population and identifying the adhesion molecules involved in their adhesion. A greater presence was observed of adherent stem cells from the marrow stroma in mononuclear cells obtained directly from the BM; a larger population of CD90+ cells in mononuclear cells from CD34-/CD45- peripheral blood with a high expression of molecules CD44 and CD62L, which suggests a greater presence of mesenchymal stem cells (MSC) in mobilized cells from the marrow stroma. The higher levels of CD34+ cells in peripheral blood stem cells with a low expression of molecules CD117- and DR- suggests the presence of hematopoietic stem cells, hemangioblasts and progenitor endothelial cells mobilized to peripheral circulation. It was found that mononuclear cells from both the BM and peripheral blood show a high presence of stem cells with expression of adhesion molecule CD44 (MMC marker), probably involved in their migration, settling and differentiation.

【 授权许可】

CC BY-NC