Diagnostic value of GATA-3 in cytological identification of parathyroid tissues

Endocrine Journal

Mitsuyoshi Hirokawa¹  Nami Takada³  Ayana Suzuki³  Seiji Kuma¹  Miyoko Higuchi³  Akira Miyauchi² 

[1] Department of Diagnostic Pathology, Kuma Hospital, Kobe, Japan;Department of Surgery, Kuma Hospital, Kobe, Japan;Department of Laboratory, Kuma Hospital, Kobe, Japan

关键词: Parathyroid;    Immunocytochemistry;    GATA-3;    Liquid-based cytology;   

DOI  :  10.1507/endocrj.EJ15-0700

学科分类：内分泌与代谢学

来源: Japan Endocrine Society

【 摘 要 】

References(32)Parathyroid and thyroid lesions appear morphologically similar in cytological smears, and their differentiation can be difficult.The purpose of this study was to determine the diagnostic value of T-cell-specific transcription factor GATA-3 as a marker of parathyroid differentiation in cytology specimens, and to examine the utility of liquid-based cytology (LBC).Cytology smears obtained from surgically removed parathyroid and thyroid specimens, including 15 normal parathyroid glands, 12 cases of parathyroid hyperplasia, 55 parathyroid adenomas, 2 follicular thyroid adenomas, and 3 papillary thyroid carcinomas, were examined by immunocytochemistry using antibodies against GATA-3, parathyroid hormone (PTH), chromogranin A, and thyroid transcription factor 1 (TTF-1).All normal and hyperplastic parathyroids and 98.2% of parathyroid adenomas were positive for GATA-3, while 33.3%, 66.7%, and 60.0% of them, respectively, were positive for PTH.The positive rates for chromogranin A among normal parathyroids (80.0%) and parathyroid adenomas (87.3%) were lower than those for GATA-3.At the same time, all thyroid-derived tumours were positive for TTF-1 and negative for GATA-3, PTH, and chromogranin A.LBC smears of 35 parathyroid lesions indicated that the positive rates for GATA-3, PTH, and chromogranin A were 97.1 %, 97.1%, and 100%, respectively, while in conventional smears, those for PTH (25.5%) and chromogranin A (78.7%) were significantly lower (p < 0.01).Our results suggest that GATA-3 is a more reliable biomarker than PTH or chromogranin A in differentiating parathyroid from thyroid lesions in cytology smears and that LBC is useful in detecting cytoplasmic antigens such as PTH and chromogranin A.

【 授权许可】

Unknown