HOMOLOGOUS DIAPHRAGM MUSCLE CONSERVED IN SUPERSATURATED SUGAR SOLUTION TO REPAR LARGE DEFECTS IN DIAPHRAGM OF DOGS

Ciência Rural

Pippi, Ney Luis²  Raiser, Alceu Gaspar²  Stedile, Rafael²  Faria, Renato Xavier²  Mazzanti, Alexandre²  Silveira, Aron Ferreira da²  Alves, Aline Souza²  Braga, Fabrício Arigony²  Gonçalves, Gentil Ferreira¹  Graça, Dominguita Lühers² 

[1] Universidade Paranaense, Umuarama;Universidade Federal de Santa Maria

关键词: implant;    muscle;    sugar;    surgery;    dog;   

DOI  :  10.1590/S0103-84782001000200014

学科分类：农业科学（综合）

来源: Universidade Federal de Santa Maria * Centro de Ciencias Rurais

【 摘 要 】

The repair induced by an homologous muscular segment conserved in 300% supersaturated sugar solution on the diaphragmatic muscle of dogs was investigated. Nine adults mongrel dogs, three males, weights varying from 10.4 to 21.0kg, were used separated in three equally numbered denominated groups I, II and III. A defect was created, on the muscular portion of the right hemidiaphragm, with dimensions of 9.0 x 6.0cm, through thoracotomy in the 10th right intercostal space. The graft was sutured with thread poligalactin 910 3-0, by means of horizontal mattress suture, in an isolated pattern. The dogs were observed for a period of 30 days (subgroups I), 75 days (groups II) and 120 days (groups III) postoperatively. After this period, macroscopic observation and collection of samples for histologic evaluation were performed. It was verified in the animals of the subgroup I, partial substitution and in the subgroups II and III, total substitution of the muscular portion of the diaphragm. A fibrovascular tissue occluding the diaphragmatic defect was observed. With the postoperative evolution, a thin tissue, almost transparent, was identified. The segment of diaphragmatic muscle conserved in 300% supersaturated sugar solution, at room temperature, for repair of great defects in the diaphragmatic muscle of dogs was substituted by a fine layer of fibrous connective tissue.

【 授权许可】

CC BY-NC