Production of genome-edited pluripotent stem cells and mice by CRISPR/Cas [Review]

Endocrine Journal

Takuro Horii¹  Izuho Hatada¹ 

[1] Laboratory of Genome Science, Biosignal Genome Resource Center, Institute for Molecular and Cellular Regulation, Gunma University, Gunma, Japan

关键词: Cas9;    CRISPR;    Embryonic stem cell;    iPS cell;    Knockout mice;   

DOI  :  10.1507/endocrj.EJ15-0734

学科分类：内分泌与代谢学

来源: Japan Endocrine Society

【 摘 要 】

References(50)Cited-By(1)Clustered regularly at interspaced short palindromic repeats (CRISPR) and CRISPR-associated (Cas) nucleases, so-called CRISPR/Cas, was recently developed as an epoch-making genome engineering technology.This system only requires Cas9 nuclease and single-guide RNA complementary to a target locus.CRISPR/Cas enables the generation of knockout cells and animals in a single step.This system can also be used to generate multiple mutations and knockin in a single step, which is not possible using other methods.In this review, we provide an overview of genome editing by CRISPR/Cas in pluripotent stem cells and mice.

【 授权许可】

Unknown