Characteristics of Prolactin Secretion in Normal and Estrogen-Treated Pituitaries of Rats at the Single Cell Level

Endocrine Journal

KAZUNORI ARITA¹  KEIICHI KAWAMOTO¹  SUMIO TAKAHASHI²  TOHRU UOZUMI¹  AKIHIRO ITO³  MASAMI YOSHIKAWA¹ 

[1] Department of Neurosurgery, School of Medicine, Hiroshima University;Department of Biology, Faculty of Science, Okayama University;Department of Cancer Research, Research Institute for Nuclear Medicine and Biology, Hiroshima University

关键词: PRL;    Anterior pituitary gland;    Estrogen;    Fischer 344 rat;    Reverse hemolytic plaque assay;   

DOI  :  10.1507/endocrj.42.235

学科分类：内分泌与代谢学

来源: Japan Endocrine Society

【 摘 要 】

References(24)Cited-By(1)We studied PRL release from individual pituitary cells in normal and estrogen (E2)-treated female Fischer 344 rats by means of a reverse hemolytic plaque assay. In this assay, the area of the hemolytic plaque around a pituitary cell is proportional to the amount of hormone secreted from the cell. Multimodal distribution of the plaque area was observed in normal pituitary cells, and was unchanged in the course of aging. In E2-treated pituitary cells, bimodal distribution was found. The heterogeneity of the distribution pattern (functional heterogeneity) and PRL secretion from a single pituitary cell decreased in association with the length of E2 treatment. The mean plaque area for the E2-treated pituitary cells was smaller than that for the normal pituitary cells. These results suggest that in E2-treated pituitary cells, the functional heterogeneity and PRL release from a single cell decrease as tumorigenesis progresses.

【 授权许可】

Unknown