Expression of PCV2 antigen in the ovarian tissues of gilts

Journal of Veterinary Medical Science

Padet TUMMARUK¹  Pachara PEARODWONG¹ 

[1] Department of Obstetrics, Gynaecology and Reproduction, Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, Thailand

关键词: follicle;    gilt;    immunohistochemistry;    ovary;    porcine circovirus type 2 (PCV2);   

DOI  :  10.1292/jvms.15-0450

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(17)The present study was performed to determine the expression of porcine circovirus type 2(PCV2) antigen in the ovarian tissue of naturally infected gilts. Ovarian tissues wereobtained from 11 culled gilts. The ovarian tissues sections were divided into two groupsaccording to PCV2 DNA detection using PCR. PCV2 antigen was assessed in the paraffinembedded ovarian tissue sections by immunohistochemistry. A total of 2,131 ovarianfollicles (i.e., 1,437 primordial, 133 primary, 353 secondary and 208 antral follicles),66 atretic follicles and 131 corpora lutea were evaluated. It was found that PCV2 antigenwas detected in 280 ovarian follicles (i.e., 239 primordial follicles, 12 primaryfollicles, 10 secondary follicles and 19 antral follicles), 1 atretic follicles and 3corpora lutea (P<0.05). PCV2 antigen was detected in primordialfollicles more often than in secondary follicles, atretic follicles and corpora lutea(P<0.05). The detection of PCV2 antigen was found mainly in oocytes.PCV2 antigen was found in both PCV2 DNA positive and negative ovarian tissues. It can beconcluded that PCV2 antigen is expressed in all types of the ovarian follicles and corporalutea. Further studies should be carried out to determine the influence of PCV2 on porcineovarian function and oocyte quality.

【 授权许可】

Unknown