Up-Regulation of JAM-1 in AR42J Cells Treated with Activin A and Betacellulin and the Diabetic Regenerating Islets

Endocrine Journal

Masashi ISSHIKI⁵  Masao OMATA²  Yukako YOSHIKUMI²  Toshiro FUJITA⁵  Yasuyuki MORISHITA¹  Junko SUZUKI²  Hideki OHNO²  Hiroshi YASUDA³  Hirohide OHNISHI⁴  Hirosato MASHIMA² 

[1] Pathology, Graduate School of Medicine, University of Tokyo;Department of Gastroenterology, Graduate School of Medicine, University of Tokyo;Division of Gastroenterology, Showa University Fujigaoka Hospital;Division of Gastroenterology and Neurology, Akita University School of Medicine;Endocrinology and Nephrology, Graduate School of Medicine, University of Tokyo

关键词: JAM-1;    AR42J cells;    Activin A;    Betacellulin;    Islet;   

DOI  :  10.1507/endocrj.K08E-017

学科分类：内分泌与代谢学

来源: Japan Endocrine Society

【 摘 要 】

References(49)Cited-By(4)Pancreatic AR42J cells demonstrate the pluripotency in precursor cells of the gut endoderm and also provide an excellent model system to study the differentiation of the pancreas. Using the mRNA differential display technique, we identified junctional adhesion molecule-1 (JAM-1), a component of the tight junction, was highly up-regulated during the differentiation of AR42J cells, although junctions were not formed. The expression level of JAM-1 showed an up-regulation in the mRNA level after 3 hours and in the protein level after 24 hours in [activin A + betacellulin]-treated AR42J cells. The expressions of its signaling molecules, PAR-3 and atypical PKCλ, also increased after the addition of activin A + betacellulin. When JAM-1 was over-expressed in [activin A + betacellulin]-treated AR42J cells, tagged-JAM-1 was observed in cytoplasm as vesicular structures and JAM-1 was colocalized with Rab3B and Rab13, members of the Rab family expressed at tight junctions. In streptozotocin-induced regenerating islets, the expression of JAM-1 was also up-regulated in the mRNA level and the protein level. JAM-1 might therefore play an important role in the differentiation of AR42J cells and the regeneration of pancreatic islets.

【 授权许可】

Unknown