Links between De Novo Fatty Acid Synthesis and Leptin Secretion in Bovine Adipocytes

Journal of Veterinary Medical Science

Hiroya KADOKAWA²  Katsuhiro AIKAWA³  Graeme B. MARTIN¹  Koji KIMURA³  Domique BLACHE¹  Ian H. WILLIAMS¹ 

[1] School of Animal Biology, Faculty of Natural & Agricultural Sciences, The University of Western Australia;Department of Veterinary Medicine, Faculty of Agricultural Science, University of Yamaguchi;Department of Animal Breeding and Reproduction, National Institute of Livestock and Grassland Science

关键词: 2-Deoxy-D-glucose;    cerulenin;    malonyl CoA;    ruminant;   

DOI  :  10.1292/jvms.69.225

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(38)Cited-By(1)Leptin secretion by adipose tissue is involved in many physiological control systems, including those that determine growth, development, body composition, milk production, and reproductive function. In the adipocyte of monogastric animals, malonyl CoA (coenzyme A) seems to link the flux of energy substrates to the control of leptin production. In this study, we tested this for ruminants by examining the effect of cerulenin, an inhibitor of de novo fatty acid synthesis at the step from malonyl CoA to palmitate, on leptin production by cultured bovine adipocytes derived from intermuscular fat. Purified preadipocytes were obtained by the ceiling culture method, and adipogenic media were used to induce their differentiation into adipocytes. We found that leptin concentrations increased significantly with time in culture, and with increases in glucose concentration. Addition of 2-deoxy-D-glucose to the medium, a competitive inhibitor of glucose transport and metabolism, suppressed leptin secretion. In media with high glucose concentrations, cerulenin enhanced leptin secretion. We conclude that, as in monogastrics, malonyl CoA may play a key role in the control of leptin secretion in ruminants.

【 授权许可】

Unknown