Effect of Trans-ε-Viniferin on In Vitro Porcine Oocyte Maturation and Subsequent Developmental Competence in Preimplantation Embryos

Journal of Veterinary Medical Science

Seong-Sung KWAK²  Yubyeol JEON²  Yeon Hee SEONG¹  Sang-Hwan HYUN²  Seung-A CHEONG² 

[1] Laboratory of Veterinary Pharmacology, College of Veterinary Medicine, Chungbuk National University, Cheongju 361–763, Republic of Korea;Laboratory of Veterinary Embryology and Biotechnology (VETEMBIO), College of Veterinary Medicine, Chungbuk National University, Cheongju 361–763, Republic of Korea

关键词: embryo;    in vitro maturation;    oocyte;    oxidative stress;    trans-ε-viniferin;   

DOI  :  10.1292/jvms.12-0105

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(53)Cited-By(3)Trans-ε-viniferin is a naturally occurring polyphenol belonging to the stilbenoid family that has been isolated from Vitis amurensis, one of the most common wild grapes in Asia. We investigated the effects of trans-ε-viniferin on in vitro maturation (IVM) and developmental competence after in vitro fertilization (IVF) or parthenogenesis (PA). We observed that trans-ε-viniferin treatment during IVM did not improve nuclear maturation rates of oocytes in any group, but significantly increased (PPP<0.05) in the 0.5 and 5.0 μM treatment groups compared with those in the control group. IVF embryos showed similar results. In conclusion, these results indicate that trans-ε-viniferin treatment during porcine IVM increased the total cell number of blastocysts, possibly by increasing intracellular GSH synthesis, reducing ROS levels, increasing DNMT1 gene expression of oocytes and decreasing pro-apoptosis gene expressions of cumulus cells.

【 授权许可】

Unknown