Evaluation of the Polymorphonuclear Cell Functions of Bottlenose Dolphins

Journal of Veterinary Medical Science

Hiroshi ASAKI²  Terumasa SHIMADA¹  Hideo AKIYOSHI¹  Mika AOKI¹  Katsura NODA¹  Fumihito OHASHI¹ 

[1] Department of Veterinary Surgery, Veterinary Science, Graduate School of Agriculture and Biological Sciences, Osaka Prefecture University;Ask Japan Co., Ltd.

关键词: dolphin;    function;    PMN;   

DOI  :  10.1292/jvms.65.727

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(11)Cited-By(10)The functions of polymorphonuclear cells (PMN) are the important non-specific defense mechanisms in the immune system. Especially marine mammals are protected by these mechanisms from the aquatic environment with a large variety of microorganisms. Therefore, we examined the PMN functions of bottlenose dolphins in order to obtain the normal ranges and to standardize the techniques. PMNs were isolated by using lymphocyte isolate solution whose density was 1.077; superoxide production was assessed by nitroblue tetrazolium reduction test (NBT) and phagocytosis was tested by using polystyrene latex beads. We showed that the optimal incubation time was 30 min in NBT assay and 12 hr in phagocytosis assay for dolphin PMNs.

【 授权许可】

Unknown