Effects of Cryopreservation on DNA Synthesis in the Inner Cell Mass of in vitro Matured/in vitro Fertilized Bovine Embryos Frozen in Various Cryoprotectants

Journal of Veterinary Medical Science

Iwao SAKONJU¹  Tatsuyuki SUZUKI¹  Mitsuhiro TAKAGI¹ 

关键词: bovine;    cryopreservation;    inner cell mass;   

DOI  :  10.1292/jvms.58.12_1237

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(12)Cited-By(5)The rate of DNA synthesis in the inner cell mass (ICM) of frozen-thawed bovine embryos was examined. Bovine blastocysts derived from in vitro matured/in vitro fertilized oocytes were frozen with 1.8 M ethylene glycol (EG), 1.6 M propylene glycol (PG) and 1.4 M glycerol plus 0.25 M sucrose (GL). Viable embryos, after thawing and culture beyond the blastocyst stage, were examined by immunocytochemical staining for detection of DNA synthesis by ICM cells. The numbers of bromodeoxyuridine-immunoreactive ICM cells of frozen-thawed embryos prepared with EG (10.7) and GL (11.5) were significantly lower than those of unfrozen embryos (17.3). The results suggest that the rates of proliferation of ICM cells of frozen-thawed bovine embryos tend to be lower than those of unfrozen embryos irrespective of the cryoprotectant used.

【 授权许可】

Unknown