Modulatory Effects of Ionized Alkali Mineral Complex (IAMC) on mRNA Expression of Porcine Cytokines

Journal of Veterinary Medical Science

Han Sang YOO¹  Sung-Jae SHIN¹  In-Soo CHOI¹ 

[1] Department of Infectious Diseases, College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University

关键词: immunomodulatory effect;    ionized alkali mineral complex;    mRNA expression;    porcine cytokine;   

DOI  :  10.1292/jvms.63.1179

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(20)Cited-By(3)It has been recognized that ionized alkali mineral complex (IAMC)-fed farm animals demonstrate higher weight gains but less incidence of diseases than the unfed ones. However, how these beneficial effects in the IAMC-fed animals are induced has not yet been elucidated clearly. In this study, porcine peripheral blood mononuclear cells (PBMC) were cultured for 4, 24, and 48 hr in the presence of IAMC, and the effects of IAMC on mRNA expression of porcine cytokines were evaluated via a reverse transcription-polymerase chain reaction (RT-PCR). Expression levels of IL-4, IL-6, and IL-10 in IAMC-treated cells were usually higher than those in the untreated ones. However, IAMC-treated cells demonstrated a reduced expression of IL-2. In addition, expression of IFN-γ was generally reduced in the cells treated with IAMC. The expression of IL-12 p35 and IL-12 p40 was not detectable in both the untreated and the IAMC-treated cells. Therefore, these results indicate that IAMC has immunomodulatory effects in vitro on the expression of porcine Th1-and Th2-type cytokines.

【 授权许可】

Unknown