The Odorant-Binding Protein Gene obp11 Shows Different Spatiotemporal Roles in the Olfactory System of Apis mellifera ligustica and Apis cerana cerana

Sociobiology

Xinnian Zeng³  Yuexiong Luo⁵  Xuefeng Zhang¹  Hongxia Zhao²  qin Liang⁴  Jianghong Lee⁴ 

[1] Guangdong Entomological Institute, Guangzhou , 510260;South china agriculture university;South China Agricultural University, Guangzhou, 510642;Fujian Agriculture and Forestry University, Fuzhou, 350002Guangdong Entomological Institute, Guangzhou , 510260;

关键词: odorant-binding protein;    olfactory;    Apis;   

DOI  :  

来源: Universidade Estadual de Feira de Santana, Brazil

【 摘 要 】

Odorant-binding proteins participate in the olfactory system of the honeybee.Apis melliferaligustica andApis ceranacerana are species of honeybee that have different biologic functions. The two species have diversified olfactory systems, withA. ceranadisplaying sensitive olfactory involvement in collecting nectar and pollen from small plants; andA. melliferacollecting from large nectariferous plants. We hypothesized that, given this difference in biologic activity, the geneobp11ofA. melliferaandA. ceranamay show different olfactory expression patterns. We cloned and sequenced theobp11genes fromA. mellifera (Amobp11)andA. cerana (Acobp11) . Using quantitative real-time PCR, we demonstrated that nurse workers, which have the highest olfactory sensitivity in theA. melliferahive, have the highest expression ofAmobp11 ; whereas 1-day-emerged  workers, which have lowest olfactory sensitivity, have correspondingly low expression. However, the highest expression ofAcobp11is observed for foragers, which display the highest olfactory sensitivity in theA. ceranapopulation. The OBP11 protein from the two species is highly conserved, with an apparent molecular weight and predicted extracellular localization that is similar to other OBP proteins. The expression of theobp11gene inA. melliferaandA. ceranacorrelates with the different roles of the olfactory system for the two different species. These findings support the critical role of odorant-binding proteins in theApisolfactory system

【 授权许可】

Unknown