Biotin-Labeled Proteins of Eimeria tenella Sporozoite

Journal of Veterinary Medical Science

Naotoshi TSUJI¹  Masataka NUMAJIRI²  Harue KASUGA-Aoki¹  Takashi ISOBE¹ 

[1] National Institute of Animal Health, 3-1-1 Kannondai, Tsukuba, Ibaraki 305-0856, Japan;Kensei Meat Inspection Center, 584 Ichinobe, Shimodate, Ibaraki 308-0827 Japan

关键词: Eimeria tenella;    sporozoite;    surface protein;   

DOI  :  10.1292/jvms.62.357

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(13)Cited-By(1)To identify surface proteins of eimerian sporozoite, Eimeria tenella sporozoites were labeled with biotin-LC-hydrazide, and analyzed by two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) and Western blotting. More than 200 sporozoite proteins were recognized with silver staining after 2D-PAGE. Among them, 7 biotin-labeled proteins were detected as surface molecules after Western blotting and reaction with horseradish peroxidase conjugated streptavidin. Their molecular masses ranged from 23 to 117 kilo daltons and their isoelectric points from 4.3 to 6.3. It appears that the biotin-labeling technique can be used to analyze of surface proteins of eimerian parasites.

【 授权许可】

Unknown