Genetic and Phylogenetic Analysis of Glycoprotein of Rabies Virus Isolated from Several Species in Brazil

Journal of Veterinary Medical Science

Samir I. SAMARA¹  Takeshi MIKAMI⁴  Darci P. NOCITI¹  Ichiro KURANE⁵  Takeo SAKAI²  Yasuo MIURA²  Fumio H. ITO³  Youko SHOJI²  Adolorata A. B. CARVALHO¹  Takuya ITOU²  Go SATO²  Mikako ITO⁵ 

[1] Faculty of Agrarian and Veterinary Science, UNESP, Jaboticabal-SP;Department of Preventive Veterinary Medicine and Animal Health, Nihon University School of Veterinary Medicine;Department of Preventive Veterinary Medicine and Animal Health, Faculty of Veterinary Medicine and Zootechny, University of São Paulo;Department of Veterinary Public Health, Nihon University School of Veterinary Medicine;Department of Virology I, National Institute of Infectious Disease

关键词: Brazil;    glycoprotein;    molecular epidemiology;    rabies virus;    vampire bat;   

DOI  :  10.1292/jvms.66.747

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(20)Cited-By(20)Genetic and phylogenetic analyses of the region containing the glycoprotein (G) gene, which is related to pathogenicity and antigenicity, and the G-L intergenic region were carried out in 14 Brazilian rabies virus isolates. The isolates were classified as dog-related rabies virus (DRRV) or vampire bat-related rabies virus (VRRV), by nucleoprotein (N) analysis. The nucleotide and amino acid (AA) homologies of the area containing the G protein gene and G-L intergenic region were generally lower than those of the ectodomain. In both regions, nucleotide and deduced AA homologies were lower among VRRVs than among DRRVs. There were AA differences between DRRV and VRRV at 3 antigenic sites and epitopes (IIa, WB+ and III), suggesting that DRRV and VRRV can be distinguished by differences of antigenicity. In a comparison of phylogenetic trees between the ectodomain and the area containing the G protein gene and G-L intergenic region, the branching patterns of the chiropteran and carnivoran rabies virus groups differed, whereas there were clear similarities in patterns within the DRRV and VRRV groups. Additionally, the VRRV isolates were more closely related to chiropteran strains isolated from Latin America than to Brazilian DRRV. These results indicate that Brazilian rabies virus isolates can be classified as DRRV or VRRV by analysis of the G gene and the G-L intergenic region, as well as by N gene analysis.

【 授权许可】

Unknown