Molecular Cloning of Novel Cytochrome P450 1A Genes from Nine Japanese Amphibian Species

Journal of Veterinary Medical Science

Kentaro Q. SAKAMOTO¹  Shoichi FUJITA¹  Mayumi ISHIZUKA¹  Shouta M.M. NAKAYAMA¹  Tomoko TANAKA-UENO² 

[1] Laboratory of Toxicology, Department of Environmental Veterinary Sciences, Graduate School of Veterinary Medicine, Hokkaido University;Center for Liberal Arts, Meiji Gakuin University

关键词: amphibian;    Anura;    cytochrome P450 1A;    phylogenetic tree;    Urodela;   

DOI  :  10.1292/jvms.001407

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(20)Cited-By(2)Novel cytochrome P450 1A (CYP1A) cDNA fragments were isolated from the liver of nine Japanese amphibian species using reverse transcription polymerase chain reaction (RT-PCR). Degenerate PCR primers were used to amplify 122-bp fragments of CYP1A cDNAs. Construction of a phylogenetic tree revealed that urodele and anuran amphibians formed two branches. Within the anuran species, three branches were formed: 1) Ranidae and Rhacophoridae, 2) Bufo japonicus formosus and Hyla japonica, and 3) Xenopus laevis. The cDNA nucleotide sequence of these CYP1A fragments showed identities ranging 72-98% (all), 72-78% (Anura vs. Urodela), 75 to 98% (Anura), 81% (Urodela), 74-80% (Xenopus laevis vs. nine Japanese amphibians).

【 授权许可】

Unknown