Yellow head virus from Thailand and gill-associated virus from Australia are closely related but distinct prawn viruses

Diseases of Aquatic Organisms

Chainarong Wongteerasupaya¹  Sakol Panyim¹  Christine M. Dimmock¹  Jeff A. Cowley¹  Peter J. Walker¹  Vichai Boonsaeng¹ 

关键词: Yellow head virus (YHV);    Gill-associated virus (GAV);    Lymphoid organ virus (LOV);    Penaeus monodon;    RT-PCR;    Sequence analysis;   

DOI  :  10.3354/dao036153

学科分类：生物科学（综合）

来源: Inter-Research

【 摘 要 】

ABSTRACT: Corresponding genomic regions of isolates of yellow head virus (YHV) from Thailand and gill-associated virus (GAV) from Australia were compared by RT-PCR and sequence analysis. PCR primers designed from sequences in the GAV ORF1b polyproteingene amplified the corresponding 577 nucleotide region of the YHV genome. Comparison of the amplified region indicated 85.1% nucleotide and 95.8% amino acid sequence identity. YHV PCR primers designed to amplify a 135 nucleotide product previouslydescribed as a YHV diagnostic probe failed to amplify the corresponding product from GAV RNA. However, the cognate GAV sequence for this and another recently reported YHV sequence were located in an upstream region of the ORF1b gene. A comparison of thesesequences indicated identities of 83.0 and 80.9% at the nucleotide level and 86.7 and 86.5% at the amino acid level, respectively. The data indicate that GAV and YHV are closely related but distinct viruses for which differential diagnostic probes can beapplied.

【 授权许可】

Unknown