Application of Real Time PCR for Diagnosis of Swine Dysentery

Journal of Veterinary Medical Science

Satoru AKASE⁵  Yoshikazu ADACHI¹  Kenji SADAMASU²  Yumi UCHITANI²  Yoshiko SOHMURA³  Keikichi TATSUTA⁴ 

[1]College of Agriculture, Ibaraki University

[2]Department of Microbiology, Tokyo Metropolitan Institute of Public Health

[3]Johnanjima Branch Office, Animal Care and Consultation Center, Bureau of Social Welfare and Public Health, Tokyo Metropolitan Government

[4]Shibaura Meat and Sanitary Inspection Station, Bureau of Social Welfare and Public Health, Tokyo Metropolitan Government

[5]Health and Safety Division, Bureau of Social Welfare and Public Health, Tokyo Metropolitan Government

关键词: Brachyspira hyodysenteriae;    NOX1;    Real time PCR;    Swine Dysentery;   

DOI  :  10.1292/jvms.71.359

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(17)Cited-By(2)Evaluation of a genetic diagnostic technique using real time PCR of Swine Dysentery (SD) was performed using nox primers. Culture, ordinary PCR and real time PCR were compared in this experiment. Sixty-seven specimens from pigs with clinical signs of SD brought to a slaughterhouse in Shibaura, Tokyo, were used. B. hyodysenteriae was isolated from 49 of the pigs, was detected by ordinary PCR in 49 of the pigs and was detected by real time PCR in 54 of the pigs. Furthermore, we were able to determine the numbers of B. hyodysenteriae cells in all positive specimens by real time PCR. The rapid diagnostic technique established in this experiment was useful for detection of B. hyodysenteriae because it was more effective than ordinary PCR and culture.

【 授权许可】

Unknown