Phloroglucinol Exerts Protective Effects Against Oxidative Stress–Induced Cell Damage in SH-SY5Y Cells

Journal of Pharmacological Sciences

Kyoung Ah Kang¹  Hye-Sun Kim²  Hee Soo Kim³  Nam Ho Lee⁴  Jin Won Hyun¹  Kihwan Lee² 

[1] College of Medicine, Jeju National University, Republic of Korea;Department of Pharmacology and Biomedical Sciences, Seoul National University College of Medicine, Republic of Korea;School of Animal Bioscience & Technology, Konkuk University, Republic of Korea;Department of Chemistry, College of Natural Sciences, Jeju National University, Republic of Korea

关键词: brown algae;    hydrogen peroxide;    phloroglucinol;    reactive oxygen species;    SH-SY5Y cell;   

DOI  :  10.1254/jphs.12056FP

学科分类：药学

来源: Nihon Yakuri Gakkai Henshuubu / Japanese Pharmacological Society

【 摘 要 】

References(26)Cited-By(7)In this study, we explored whether phloroglucinol (1,3,5-trihydroxybenzene) exerts protective effects against oxidative stress–induced cytotoxicity in SH-SY5Y cells, a neuroblastoma cell line. The central nervous system is especially vulnerable to oxidative stress because the brain consumes large amounts of energy to perform its complicated functions, but contains a lower level of anti-oxidant enzymes compared with other peripheral tissues. In SH-SY5Y cultures, pretreatment with 10 μg/ml of phloroglucinol attenuates the cytotoxicity of hydrogen peroxide. In addition, when assessed with 2′,7′-dichlorofluorescein diacetate dye, the increase in reactive oxygen species (ROS) induced by hydrogen peroxide was significantly reduced by pretreatment with phloroglucinol. ROS is known to cause lipid peroxidation of the cell membranes, the carbonylation of intracellular proteins, and DNA damage, which can lead to various cellular dysfunctions and eventually cellular death. We found that pretreatment with phloroglucinol down-regulated the levels of 8-isoprostane, protein carbonylation, and 8-hydroxy deoxyguanine caused by hydrogen peroxide treatment in SH-SY5Y cells. These results indicate that phloroglucinol exerts protective effects against oxidative stress-induced cell damage in SH-SY5Y cells.

【 授权许可】

Unknown