Hlcyst-1 and Hlcyst-2 are Potential Inhibitors of HlCPL-A in the Midgut of the Ixodid Tick Haemaphysalis longicornis

Journal of Veterinary Medical Science

Takeharu MIYOSHI¹  Takeshi HATTA¹  Naotoshi TSUJI¹  Kozo FUJISAKI³  Shiro KUSHIBIKI²  Kayoko YAMAJI²  ANISUZZAMAN¹  M. Abdul ALIM¹ 

[1] National Institute of Animal Health, National Agriculture and Food Research Organization;Graduate School of Life and Environmental Sciences, University of Tsukuba;Department of Frontier Veterinary Medicine, Kagoshima University

关键词: arthropods;    enzymes;    tick;    vector biology;   

DOI  :  10.1292/jvms.09-0561

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(28)Cited-By(10)Although the actions of cysteine proteases are controlled in part by endogenous tight-binding cysteine protease inhibitors from the cystatin superfamily, regulatory mechanisms used by ticks to control protease activities are unknown. We report here the interaction of 2 endogenous midgut cysteine protease inhibitors, Hlcyst-1 and Hlcyst-2, with an endogenous midgut cysteine protease, HlCPL-A in Haemaphysalis longicornis. In vitro inhibition assays demonstrated that the hydrolytic activity of HlCPL-A was inhibited by Hlcyst-1 and Hlcyst-2 in dose dependent manner. Immunofluorescent studies revealed that Hlcyst-1 and Hlcyst-2 are co-localized with HlCPL-A in the epithelial cells of the midgut. The hemoglobin degradation activity of HlCPL-A was dose-dependently inhibited by Hlcyst-1 and Hlcyst-2. These results strongly indicate that, Hlcyst-1 and Hlcyst-2 are possible inhibitor of HlCPL-A and play a key role in regulatory mechanisms of hemoglobin degradation process in ticks.

【 授权许可】

Unknown