Confirmation of crayfish plague in Italy: detection of Aphanomyces astaci in white clawed crayfish

Diseases of Aquatic Organisms

Alessandra Paolini¹  Daniela Zezza¹  Luciano Ricchiuti¹  Maurilia Marcacci¹  Nicola Ferri¹  Rossella Lelli¹  Cesare Camm๠

关键词: Crayfish plague;    Aphanomyces astaci;    Aphanomyces repetans;    Austropotamobius pallipes;    PCR;    Culture method;   

DOI  :  10.3354/dao02207

学科分类：生物科学（综合）

来源: Inter-Research

【 摘 要 】

ABSTRACT: In the summer of 2009, high levels of mortality among white clawed crayfish Austropotamobius pallipes were observed in 3 watercourses of central Italy. PCR and culture methods were used to detect the causative agent of the disease. Two strains of Aphanomyces spp. were isolated and identified by PCR and DNA sequencing as Aphanomyces astaci and A. repetans. This is the first crayfish plague outbreak in Italy to be confirmed by the isolation in culture of a pathogen from Austropotamobius pallipes.

【 授权许可】

Unknown