Sensitive Detection of Whole-Genome Differentiation among Closely-Related Species of the Genus Fusarium Using DNA-DNA Hybridization and a Microplate Technique

Journal of Veterinary Medical Science

Yukiko HARA-KUDO¹  Yoshiko SUGITA-KONISHI¹  Maiko WATANABE¹  Keiichi GOTO²  Yoichi KAMATA¹ 

[1] Division of Microbiology, National Institute of Health Sciences, 1–18–1 Kamiyoga, Setagaya-ku, Tokyo 158–8501, Japan;Food Research Laboratories, Mitsui Norin Co., Ltd., 223–1 Miyabara, Fujieda, Shizuoka 426–0133, Japan

关键词: chemiluminescence;    digoxigenin;    DNA-DNA hybridization;    Fusarium;    microplate technique;   

DOI  :  10.1292/jvms.12-0034

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(20)Cited-By(1)We developed a new system for detection of whole-genome differentiation using DNA-DNA hybridization, and tested its sensitivity with three closely-related Fusarium species. We compared DNA-DNA relatedness to nucleotide sequence homologies of five genetic regions between each of five strains of three Fusarium species. DNA-DNA relatedness by our system was 16.2–86.6%. Sequence homologies of 18S rDNA, rDNA cluster region from ITS1 to 28S rDNA, β-tub, EF-1α and lys2 were 100.0, 99.0–100.0, 96.7–100.0, 95.1–99.4, and 94.7–100.0%, respectively. Our system could clearly detect differentiation between closely-related fungal species which have very similar morphological-characteristics, and exhibit little diagnoses in nucleotide sequences. Our results suggest that this system is a good tool for identification and phylogenetic analysis of closely-related fungal species.

【 授权许可】

Unknown