Production and Characterization of Monoclonal Antibodies against Porcine Interleukin-4

Journal of Veterinary Medical Science

Hirotomo KATO¹  Tomomi HASHIMOTO¹  Yoji ISHIKURA¹  Yorihiro NISHIMURA¹  Hiroyuki IWATA¹ 

[1] Department of Veterinary Hygiene, Faculty of Agriculture, Yamaguchi University

关键词: IL-4;    monoclonal antibody;    porcine;    recombinant;   

DOI  :  10.1292/jvms.67.503

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(20)Interleukin 4 (IL-4) is an important regulatory cytokine produced by activated T lymphocytes and mast cells, and regulates the growth and differentiation of cells such as B and T lymphocytes. In the present study, recombinant thioredoxin (Trx)-porcine IL-4 (pIL-4) fusion protein was prepared by Escherichia coli (E. coli), and by using this protein as an immunogen, monoclonal antibodies (mAbs) against pIL-4 were produced to establish a basis for a research on immune responses in pigs. Six stable hybridoma cell lines were successfully established and specific binding of each mAb to recombinant pIL-4 produced by E. coli and insect cells infected with recombinant baculovirus was shown by enzyme-linked immunosorbent assay (ELISA) and/or immunoblot analysis. Isotype analyses of these mAbs revealed that the subclass of 5 out of 6 mAbs was IgG1 and the rest was IgG2b. Further, assessment of their epitopes by competition binding assay indicated that the mAbs obtained in this study bound to 4 different epitopes. The recombinant proteins and mAbs produced in this study will be useful tools for the assessment of porcine immune system.

【 授权许可】

Unknown