Changes in Interleukin-1β mRNA Expression in the Rat Ovary during the Estrous Cycle in Response to Lipopolysaccharide

Endocrine Journal

HIROSHI KATO¹  SHUJI TAKIGUCHI¹  YOSHIAKI YAMAGATA¹  SHIRO KASHIDA¹  YASUHIKO NAKAMURA¹  HIROSHI TAMURA¹  NORIHIRO SUGINO¹ 

[1] Department of Obstetrics and Gynecology, Yamaguchi University School of Medicine

关键词: Estrous cycle;    Interleukin-1;    Lipopolysaccharide;    Macrophage;    Rat;   

DOI  :  10.1507/endocrj.46.293

学科分类：内分泌与代谢学

来源: Japan Endocrine Society

【 摘 要 】

References(29)Cited-By(5)The changes in interleukin-1(IL-1)β mRNA expression and the number of macrophages were studied in the ovary during the estrous cycle in rats and after intraperitoneal injection of lipopolysaccharide (LPS, 2mg/body )2 hours before autopsy. IL-1β mRNA expression was very low in the ovary, and there was no statistically significan tchange during the estrous cycle. Hybridization signals of IL-1β mRNA were localized intensely in the thecal layer, moderately in the corpora lutea, and slightly in granulosa cells of the ovary during the cycle. The number o fmacrophages seen mainly in the hilum and interstitium significantly increased on proestrus compared with otherestrous days. LPS significantly increased IL-1β mRNA expression on each day with the highest response to LPS at 1500h on proestrus, and caused an increase in the number of macrophages in the ovary within 2 hours. These results indicate that IL-1β mRNA expressions are low during the estrous cycle in rats, and proestrus is the day of maximal IL-1β synthesis in response to LPS. The increase in IL-1β synthesis caused by LPS might be due to at least the influx of macrophages into the ovary.

【 授权许可】

Unknown