Glycated Hemoglobin Fractions in Normal and Diabetic Dogs Measured by High Performance Liquid Chromatography

Journal of Veterinary Medical Science

Toshinori SAKO¹  Hidekazu KOYAMA¹  Shinogu HASEGAWA¹  Naoyuki TAKEMURA¹  Shigekatsu MOTOYOSHI¹ 

关键词: diabetes;    dog;    glycated hemoglobin;    HPLC.;   

DOI  :  10.1292/jvms.53.65

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(21)Cited-By(6)We established a new analytical condition to measure the canine glycated hemoglobin by high performance liquid chromatography (HPLC) using cation exchange column. The canine hemoglobin gave five peaks consisting of 2 major and 3 minor hemoglobin fractions such as HbA1a, HbA1b and HbA1c. Measurement was done in 38 clinically normal dogs and 10 diabetic dogs. Mean HbA1c values (% of total Hb) in normal and diabetic dogs were 2.60 and 6.41%, respectively. And mean HbA1 values were 3.58 and 7.41%, respectively. The mean values of the canine HbA1c and HbA1 in diabetic dogs was higher than those in normal dogs, significantly (p<0.01). Advantages of the HPLC method and applicability for monitoring effectiveness of insulin therapy in the canine diabetes mellitus are discussed.

【 授权许可】

Unknown