Intrauterine embryo transfer with canine embryos cryopreserved by the slowfreezing and the Cryotop method

Journal of Veterinary Medical Science

Taku KIMURA¹  Tatsuya HORI¹  Masanori KOBAYASHI¹  Toshihiko TSUTSUI¹  Eiichi KAWAKAMI¹  Hitoshi USHIJIMA² 

[1] Laboratory of Reproduction, Nippon Veterinary and Life Science University, 1�?7�?1 Kyonan-cho, Musashino-shi, Tokyo 180�?8602, Japan;Laboratory of Animal Reproduction, Nippon Veterinary and Life Science University, 1�?7�?1 Kyonan-cho, Musashino-shi, Tokyo 180�?8602, Japan

关键词: cryopreservation;    Cryotop method;    dog;    embryo;    slow freezing method;   

DOI  :  10.1292/jvms.16-0037

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(23)Canine embryos (8-cell to blastocyst stages) frozen-thawed using the slow-freezing methodwith glycerol (four recipients) or dimethyl sulfoxide (three recipients) as acryoprotectant and vitrified-warmed using the Cryotop method (five recipients) weresurgically transferred into the unilateral uterine horn of recipient bitches. As a result,the morphology of embryos frozen-thawed using the slow-freezing method was judged to benormal, but no conception occurred in any of the recipient bitches. Two of the fivebitches that received transferred embryos (morula to early blastocyst stages)vitrified-warmed using the Cryotop method became pregnant and produced normal pups (1/9embryos, 11.1% and 1/6 embryos, 17.0%). It was concluded that the Cryotop method was moreappropriate for canine embryo cryopreservation than the slow-freezing method, which isused for the cryopreservation of embryos of other mammalian species.

【 授权许可】

Unknown