期刊论文详细信息
Diseases of Aquatic Organisms
In vitro quenching of fish pathogen Edwardsiella tarda AHL production using marine bacterium Tenacibaculum sp. strain 20J cell extracts
Manuel Romero1  Celia Mayer1  Noemí Buján1  Andrea Muras1  Ana Otero1  Beatriz Magariños1 
关键词: Edwardsiella tarda;    Quorum sensing;    Quorum quenching;    N-acylhomoserine lactone;    AHL;    Lactonase;    Tenacibaculum;    Cytophaga–Flexibacter–Bacteroidetes;    CFB;   
DOI  :  10.3354/dao02697
学科分类:生物科学(综合)
来源: Inter-Research
PDF
【 摘 要 】

ABSTRACT: Quorum quenching (QQ) has become an interesting alternative for solving the problem of bacterial antibiotic resistance, especially in the aquaculture industry, since many species of fish-pathogenic bacteria control their virulence factors through quorum sensing (QS) systems mediated by N-acylhomoserine lactones (AHLs). In a screening for bacterial strains with QQ activity in different marine environments, Tenacibaculum sp. strain 20J was identified and selected for its high degradation activity against a wide range of AHLs. In this study, the QQ activity of live cells and crude cell extracts (CCEs) of strain 20J was characterized and the possibilities of the use of CCEs of this strain to quench the production of AHLs in cultures of the fish pathogen Edwardsiella tarda ACC35.1 was explored. E. tarda ACC35.1 produces N-hexanoyl-L-homoserine lactone (C6-HSL) and N-oxohexanoyl-L-homoserine lactone (OC6-HSL). This differs from profiles registered for other E. tarda strains and indicates an important intra-specific variability in AHL production in this species. The CCEs of strain 20J presented a wide-spectrum QQ activity and, unlike Bacillus thuringiensis serovar Berliner ATCC10792 CCEs, were effective in eliminating the AHLs produced in E. tarda ACC35.1 cultures. The fast and wide-spectrum AHL-degradation activity shown by this member of the Cytophaga–Flexibacter–Bacteroidetes group consolidates this strain as a promising candidate for the control of AHL-based QS pathogens, especially in the marine fish farming industry.

【 授权许可】

Unknown   

【 预 览 】
附件列表
Files Size Format View
RO201911300640998ZK.pdf 8KB PDF download
  文献评价指标  
  下载次数:28次 浏览次数:17次