A Real-Time PCR for Detection and Quantification of Mycoplasmaovipneumoniae

Journal of Veterinary Medical Science

Falong YANG¹  Hua YUE¹  Xiaonong YANG¹  Xiaofang DAO¹  Alex RODRIGUEZ-PALACIOS²  Xufei FENG¹  Cheng TANG¹ 

[1] Department of Veterinary Medicine, College of Life Science and Technology, Southwest University for Nationalities, Chengdu 610041, China;Division of Gastroenterology and Liver Disease, Case Western Reserve University, Cleveland, OH 44106, U.S.A.

关键词: Mycoplasma ovipneumoniae;    quantification;    real-time PCR;   

DOI  :  10.1292/jvms.14-0094

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(17)Cited-By(2)A real-time PCR for detectionand quantification of M. ovipneumoniae was developed using 9 recentlysequenced M. ovipneumoniae genomes and primers targeting a putativeadhesin gene p113. The assay proved to be specific and sensitive (with adetection limit of 22 genomic DNA) and could quantify M. ovipneumoniaeDNA over a wide linear range, from 2.2 × 102 to 2.2 × 107genomes.

【 授权许可】

Unknown