Transmission of iridovirus from freshwater ornamental fish (pearl gourami) to marine fish (rock bream)

Diseases of Aquatic Organisms

Joon Bum Jeong¹  Lyu Jin Jun¹  Hye Jin Cho¹  Joon-Ki Chung¹  Hyun Do Jeong¹  Su Hee Hong¹ 

关键词: Cross-infection;    Cohabitation;    Megalocytivirus;    Real-time PCR;    Pearl gourami;    Trichogaster leeri;    Rock bream;    Oplegnathus fasciatus;   

DOI  :  10.3354/dao01961

学科分类：生物科学（综合）

来源: Inter-Research

【 摘 要 】

ABSTRACT: Freshwater pearl gourami Trichogaster leeri and seawater rock bream Oplegnathus fasciatus infected by the iridoviruses PGIV-SP and IVS-1 were carrying similar numbers of viral particles (2.52 × 10⁸ and 2.46 × 10⁸ viral genome copies mg^–1 spleen tissue, respectively). The viral genome copy number for both iridoviruses decreased much faster in seawater than in freshwater, reaching a concentration of less than 0.5%, versus 26 to 54% in freshwater, after 4 d of incubation at 25°C. The decrease in copy number altered the infectivity of the viruses, as reflected by the decreased cumulative mortality of rock bream injected intraperitoneally with the incubated iridoviruses. Moreover, uninfected rock bream cohabitated with PGIV-SP-challenged rock bream showed 100% cumulative mortality; a similar experiment using IVS-1 had the same result, implying the potential for iridoviral transmission from freshwater ornamental fish to marine fish even in a marine environment. Of 58 outwardly healthy marine fish groups collected from various markets, 2 rock bream groups and 1 sea perch group Lateolabrax sp. tested positive for PGIV-SP by 2-step polymerase chain reaction (PCR). Thus, PGIV-SP from freshwater ornamental fish may have crossed both environmental and species barriers to infect marine fish such as rock bream.

【 授权许可】

Unknown