Acute Hepatitis in a Piglet Experimentally Inoculated with Tissue Homogenates from Pigs with Postweaning Multisystemic Wasting Syndrome

Journal of Veterinary Medical Science

Takuya HIRAI¹  Tetsuo KATO¹  Kazumoto SHIBUYA¹  Kouichi KUSANAGI¹  Tetsuo NUNOYA¹  Takeshi IHARA¹ 

[1] Nippon Institute for Biological Science

关键词: hepatitis;    PCV-2;    PMWS;   

DOI  :  10.1292/jvms.65.1041

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(14)Cited-By(4)Five 2 day-old colostrum-deprived piglets were inoculated with tissue homogenates from pigs with postweaning multisystemic wasting syndrome. One of the five piglets developed icterus and died 23 days post-inoculation. Histologic examination revealed acute hepatitis. Porcine circovirus type 2 (PCV-2) antigen and nucleic acid were detected in hepatocytes and phagocytic cells. Ultrastructurally, hepatocytes and phagocytic cells had large numbers of cytoplasmic inclusions, which were composed of electron-dense paracrystalline arrays of small non-enveloped viral particles approximately 17 nm in diameter. Apoptotic hepatocytes were confirmed by the TUNEL method and electron microscopic examination. These findings may indicate that hepatocellular necrosis is associated with replication of PCV-2. Apoptosis of hepatocytes also contributes to the pathogenesis of hepatic lesions in this case.

【 授权许可】

Unknown