Various Emetogens Increase the Secretion of Salivary Amylase in Rats: a Potential Model in Emesis Research

Journal of Pharmacological Sciences

Eri Miwa¹  Harumi Kitaura¹  Hatsue Furukawa¹  Takako Iwachido¹  Hideo Fukui¹ 

[1] Development Research Center, Takeda Pharmaceutical Company, Ltd., Japan

关键词: rat salivary amylase activity;    vomiting;    emesis;    cisplatin;   

DOI  :  10.1254/jphs.10037FP

学科分类：药学

来源: Nihon Yakuri Gakkai Henshuubu / Japanese Pharmacological Society

【 摘 要 】

References(43)Cited-By(2)We investigated the effects of various emetic agents: cisplatin, apomorphine, lithium chloride (LiCl), rolipram, sibutramine, and the β3-adrenoceptor (AR) agonist CL316243 on salivary amylase secretion in rats. We also determined the inhibitory effect of granisetron, a 5-HT3–receptor antagonist, on cisplatin-induced increased salivary amylase activity and the inhibitory effect of bilateral abdominal vagotomy on increases in salivary amylase activity induced by cisplatin and LiCl. Granisetron was administered 15 min before and 1 h after cisplatin administration. Cisplatin (10 – 15 mg/kg, i.v.) increased salivary amylase activity dose-dependently and induced an acute increase from 1.5 h post-treatment with 15 mg/kg. Apomorphine (1 – 3 mg/kg, s.c.), LiCl (120 mg/kg, i.p.), rolipram (3 – 10 mg/kg, p.o.), and sibutramine (10 mg/kg, p.o.) induced significant increases in salivary amylase secretion. On the other hand, CL316243 did not stimulate salivary amylase secretion. The increased amylase activity induced by cisplatin (15 mg/kg, i.v.) was inhibited significantly by granisetron (1 or 3 mg/kg × 2, i.v.) or tended to be inhibited by bilateral abdominal vagotomy, whereas an increase in amylase activity produced by LiCl was not inhibited by abdominal visceral nerve section. These results suggest that salivary amylase activity is useful as a marker for emesis in rats, a species that does not exhibit vomiting.

【 授权许可】

Unknown