Immunohistochemical Detection of Phosphatidylserine and Thrombospondin on Denucleating Erythroblasts in Rat Bone Marrow

Journal of Veterinary Medical Science

Eiichiro TAKAHARA¹  Takanori MIKI³  Yoshiki TAKEUCHI³  Takahiko ETOH²  Yoshiki MATSUMOTO³  Wang-Mei QI²  Nobuhiko HOSHI¹  Kankanam Gamage Sanath UDAYANGA¹  Hiroshi KITAGAWA¹  Katsuhiko WARITA³  Toshifumi YOKOYAMA¹ 

[1] Department of Bioresource Science, Graduate School of Agricultural Science, Graduate School of Science and Technology, Kobe University;Department of Bioresource and Agrobiosciences, Graduate School of Science and Technology, Kobe University;Department of Anatomy and Neurobiology, Faculty of Medicine, Kagawa University

关键词: apoptosis;    denucleation;    erythropoiesis;    phosphatidylserine;    thrombospondin;   

DOI  :  10.1292/jvms.10-0437

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(24)The hypothesis that apoptotic factors play some roles in the denucleation of erythroblasts has been confirmed by the immunohistological detection of both phosphatidylserine and thrombospondin as phagocytosis-inducing factors in general apoptotic events. Both phosphatidylserine and thrombospondin were detected on the surface of cell membrane of mature erythroblasts, while thrombospondin was also detected in more immature erythroblasts. The intensities of their immune reactions increased as the erythroids matured. During denucleation, the positivities of both phosphatidylserine and thrombospondin were restricted on the surface of the cell membrane surrounding the protruding nuclei. Thus, the apoptotic process involves denucleation of erythroblasts and phosphatidylserine, and thrombospondin acts as phagocytosis-inducing factors in the denucleation event.

【 授权许可】

Unknown