Characterization of an iridovirus from the cultured pig frog Rana grylio with lethal syndrome

Diseases of Aquatic Organisms

Qi-Ya Zhang¹  Jinghe Mao¹  Jian-Fang Gui¹  Zhen-Qiu Li¹  V. Gregory Chinchar¹  Feng Xiao¹ 

关键词: Rana grylio;    Iridovirus;    FV3;    Ranavirus;    Viral disease;   

DOI  :  10.3354/dao048027

学科分类：生物科学（综合）

来源: Inter-Research

【 摘 要 】

ABSTRACT: Three virus isolates, RGV-9506, RGV-9807 and RGV-9808, were obtained from cultured pig frogs Rana grylio undergoing lethal infections. Previously, the first isolate, RGV-9506, was shown to be an iridovirus based on ultrastructural andmorphological studies. In the present study, the original isolate, along with 2 recent ones, were more extensively characterized by experimental infection studies, histopathology, electron microscopy, serological reactivity, gel electrophoresis of viralpolypeptides and DNA restriction fragments, PCR amplification, and nucleic acid sequence analysis of the major capsid protein (MCP) gene. The 3 isolates were shown to be identical to each other, and very similar to FV3, the type species of the genusRanavirus (family Iridoviridae). These results suggest that RGV should be considered a strain of FV3, and indicate that FV3-like iridoviruses are capable of causing widespread, severe disease among cultured frogs.

【 授权许可】

Unknown