Detection and characterization of a Cucumber mosaic virus isolate infecting peperina, a species native to Argentina

Agriscientia

Di Feo, L¹  Rodríguez Pardina, P¹  Ojeda, M²  Biderbost, E¹ 

[1] INTA, Argentina;Universidad Nacional de Córdoba, Argentina

关键词: Cucumovirus;    Mynthostachys mollis;    RT-PCR sequenceDetección y caracterización de un aislamiento de Cucumber mosaic virus que infecta peperina;    una especie nativa de Argentina;   

DOI  :  

学科分类：农业科学（综合）

来源: Universidad Nacional de Cordoba * Facultad de Ciencias Agropecuarias

【 摘 要 】

Minthostachys mollis (Kunth.) Griseb., "peperina", un miembro de la familia Lamiaceae, es una especie aromática que se emplea en la farmacología moderna y en medicina. Está ampliamente distribuida en los Andes, desde Venezuela y Colombia hasta Argentina. En el último país, la principal área de explotación de peperina incluye el área serrana de la provincia de Córdoba, donde la especie es arrancada indiscriminadamente, lo que conlleva una pérdida irreversible de germoplasma. A los fines de preservar este recurso nativo y fuente regional de ingresos, la especie está siendo domesticada. Durante este proceso, se observó la aparición de síntomas de un conspicuo mosaico amarillo, típico de infección viral. Análisis biológicos, serológicos y moleculares (RT-PCR, RFLP, clonado y secuenciación) pusieron de manifiesto la presencia del subgrupo IA de Cucumber mosaic virus en las plantas domesticadas de peperina. El aislamiento viral estudiado está íntimamente relacionado con la raza Y previamente informada en Japón. Éste es el primer informe de un virus que infecta a la peperina. Palabras claves: Cucumovirus; Mynthostachys mollis; Secuencia producto de RT-PCRFecha de recepción: 25/06/12;Fecha de aceptación: 22/10/13  INTRODUCTIONMinthostachys mollis (Kunth.) Griseb., locally known as "peperina", is a member of the family Lamiaceae; it is a perennial, aromatic semi-shrub of about 0.3-2.0 m in height. Due to its menthol content, its aroma resembles that of mint. It is widely used in infusions because of its stimulant, antispasmodic, antidiarrhoea, anticholera, antiemetic and digestive properties (Ordóñez et al., 2006). This aromatic plant is also used for liquor and mixed herb production. It has received increasing attention from modern pharmacology and medicine, as plant decoctions and extracted essential oils are tested for pharmacological effects (Ojeda et al., 2004; Ordóñez et al., 2006; Banchio et al., 2007). This species is widely distributed in the Andes, from Venezuela and Colombia to Argentina. In Argentina, it is present in the north-western region (provinces of Salta, Jujuy, Catamarca, Tucumán, and La Rioja) and in the central-western region (San Luis and Córdoba) (Schmidt-Lebuhn et al., 2008). In Córdoba, the plant grows between 700 and 1200 m a.s.l. In this area, the trade of these plants reaches its maximum levels; hence, it is indiscriminately gathered by collectors, who sometimes uproot whole plants without considering the phenological state or the ability of the species to recover. This practice has a strong impact on natural plant resources, modifies the environment, and leads to irrecoverable loss of germplasm through genetic erosion. For these reasons, research is carried out aiming at conservation, domestication and culture improvement of this native species. However, during the domestication process, loss of resistance genes takes place, as demonstrated for example in rice (Hiroaki & Takeshi, 2010). Peperina plants were asymptomatic at their natural habitat (Biderbost, unpublished data). However, when they were transferred to the experimental plots, conspicuous yellow mosaic symptoms, typical of virus infection, started to appear.Cucumber mosaic virus (CMV), a member of the family Bromoviridae, and the type member of the genus Cucumovirus, is one of the most important viruses in the world, infecting more than 1200 species in over 100 families of mono and dicotyledonous plants, including vegetables, ornamentals, legumes and other important crops (Palukaitis & Garcia-Arenal, 2003). The virus is mechanically transmitted by sap and naturally spread by more than 80 aphid species in a non-persistent manner (García-Arenal et al., 2000). The virus genome consists of three positive-sense, single-stranded RNAs (RNA 1, RNA2 and RNA3) (Owen & Palukaitis, 1988). Many CMV strains have been described and classified into subgroups I and II on the basis of serology, nucleic acid hybridization, and restriction fragment length polymorphism (Owen & Palukaitis, 1988; Hu et al., 1995; Finetti Sialer et al., 1999; Szilassy et al., 1999). Subgroup I is further subdivided into IA and IB on the basis of gene sequence and phylogenetic analysis (Roossinck, 2002). The objective of the present work was to identify and characterize the possible viral agents infecting peperina in Argentina. MATERIALS AND ME

【 授权许可】

CC BY-NC