The Ovario-Protective Effect of Erythropoietin against Oxidative Damage Associated with Reperfusion Following Ovarian Torsion in Rat | Science Publications

American Journal of Animal and Veterinary Sciences

K.-S. Maryamalsadat¹  S.-R. Jafar¹  R. Mohammad-Reza¹  G. Morteza¹  R. Leila¹  R. Nadereh¹  S.-M. Manizheh¹  O. Elaheh¹  M. Omid¹ 

关键词: Ovarian torsion;    Analysis Of Variance (ANOVA);    Malondialdehyde (MDA);    oxidative;    cytokine;    Superoxidase (SOD);    ischemic effect;   

DOI  :  10.3844/ajavsp.2011.18.24

学科分类：兽医学

来源: Science Publications

【 摘 要 】

Problem statement: To show the effect of recombinant Erythropoietin (rhEPO) on theovarian viability and histology in the twisted ischemic ovaries in rats, followed by detorsion.Approach: An experimental study was conducted in primate clinic of the School of Medicine, TabrizUniversity of Medical Sciences from Dec. 2008 to Apr. 2009. Forty, 4 month old Wistar rats are casedin the present study divided into 4 groups. Ovarian ischemia was performed by torsion which was keptstable by using a vascular microclip for 4 h. In group 1, the ovary were surgically removed, fixed andanalyzed histochemically. In group 2, the same procedure was repeated after 3 h reperfusion. In thenext two groups, the same was performed rhEPO was administrated 400 u kg⁻¹ 1 h after torsion of theovaries. Results: Thirty-two out of 40 rats were followed. There was a significant difference betweengroups in the levels of Malondialdehyde (MDA), Total Antioxidants (TA), Superoxidase (SOD), NitricOxide (NO) glutathione ( p<0.001). Addition of rhEPO maintained the normal histologic appearance ininterventional groups compared with controls. Conclusion: Administration of rhEPO was effectivein reducing the ischemic effect and free radicals damages of ovarian torsion in rats.

【 授权许可】

Unknown