Ghrelin Gene Expression in Broiler Proventriculus Tissue are Changed by Feed Restriction, Different Dietary Energy and Protein Levels | Science Publications

American Journal of Animal and Veterinary Sciences

Mojtaba Tahmoorespur¹  Abdolreza Salehi¹  Karim Nobari¹  Shokoufe Ghazanfari¹  Mohammad R. Nassiry¹ 

关键词: Feed restriction;    energy;    protein and ghrelin gene expression;   

DOI  :  10.3844/ajavsp.2010.175.179

学科分类：兽医学

来源: Science Publications

【 摘 要 】

Problem statement: The aim of this study was to investigate the effects of feed restrictionand different energy and protein contents of diet on ghrelin gene expression in broiler chicken.Approach: Feeding programs consisted of ad libitum and feed restriction, two energy levels (3100 and2800 kcal ME kg^-1) and three protein levels (22.3, 19.3 and 16.3% CP). Feed restriction was appliedduring 22-32 days of age. Proventriculus samples were collected at 21, 32 and 49 days of age. GhrelinmRNA expression in proventriculus tissue was quantitate using Real Time quantitative PCR. Results: Wefound that ghrelin gene expression was increased in restricted chicks compared with those fed adlibitum at 32 days of age (p = 0.09) but feed restriction had no effect on ghrelin gene expression at 49days of age. A positive response in ghrelin gene expression was achieved by decreasing energy level inthe diet at 21 days of age (p<0.07) and at 32 days of age (p<0.05). Also, we showed that dietary proteinhad no effect on ghrelin gene expression, whereas there was a tendency for an increase in ghrelin geneexpression as protein decreased at total period. Conclusion: The present study, we investigated theeffects of feed restriction and different energy and protein contents of the diet on ghrelin geneexpression in broiler chicken. We have characterized chicken ghrelin cDNA in proventriculus tissue inbroiler chicken. We also found that ghrelin gene expression is differently suppressed by dietmanipulations. Additional studies are necessary to investigate the role of nutrition on ghrelin geneexpression in proventriculus tissue in broiler chicken.

【 授权许可】

Unknown