Libyan Journal of Medicine | |
Potent hepatoprotective effect in CCl4-induced hepatic injury in mice of phloroacetophenone fromMyrcia multiflora | |
Karina Bettega Felipe3  Jo茫o Francisco Gomes Correia3  Eduardo Cargnin-Ferreira3  Danilo Wilhelm Filho2  Eduardo Antonio Ferreira3  Eliana Fortes Gris3  Rozangela Curi Pedrosa1  | |
[1] Universidade Federal de Santa Catarina;niversity Federal of Santa Catarina;University Federal of Santa Catarina | |
关键词: Antioxidant; Hepatoprotective; 2; 4; 6-trihydroxiacetophenone; Myrcia multiflora; CCl4; Silymarin; | |
DOI : | |
学科分类:医学(综合) | |
来源: Co-Action Publishing | |
【 摘 要 】
Background: This study investigated the hepatoprotective effect and antioxidant properties of phloroacetophenone (2',4',6'-trihydroxyacetophenone - THA), an acetophenone derived from the plant Myrcia multiflora. Material & Method: The free radical scavenging activity in vitro and induction of oxidative hepatic damage by carbon tetrachloride (CCl 4 ) (0.5 ml/kg, i.p.) were tested in male Swiss mice (25±5 g). Results: This compound exhibited in vitro antioxidant effects on FeCl 2 -ascorbate-induced lipid peroxidation (LPO) in mouse liver homogenate, scavenging hydroxyl and superoxide radicals, and 2,2-diphenyl-1- picrylhydrazyl. The in vivo assays showed that THA significantly (p<0.01) prevented the increases of hepatic LPO as measured by the levels of thiobarbituric acid-reactive substances, mitochondrial swelling. It also protected hepatocytes against protein carbonylation and oxidative DNA damage. Consistent with these observations, THA pre-treatment normalized the activities of antioxidant enzymes, such as catalase, glutathione peroxidase, and superoxide dismutase, and increased the levels of reduced glutathione (GSH) in CCl 4 -treated mice. In addition, THA treatment significantly prevented the elevation of serum enzymatic activities of alanine amino transferase, aspartate amino transferase, and lactate dehydrogenase, as well as histological alterations induced by CCl 4 . Silymarin (SIL) (24 mg/kg), a known hepatoprotective drug used for comparison, led to a significant decrease (p<0.01) in activities of theses enzymes in way very similar to that observed in pre-treatment with THA. Conclusion: These results suggest that the protective effects are due to reduction of oxidative damage induced by CCl 4resulting from the antioxidant properties of THA. Keywords: antioxidant; hepatoprotective; 2',4',6'-trihydroxyacetophenone; Myrcia multiflora; CCl 4 ; Silymarin (Published: 8 June 2010) Citation: Libyan J Med 2010, 5: 4891 - DOI: 10.3402/ljm.v5i0.4891
【 授权许可】
Unknown
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