Detection of DNA Hypomethylation Mediated Floral Induction in Longan and Spinach Using the HAT-RAPD Technique| Science Publications

American Journal of Applied Sciences

R. Chundet¹  R. W. Cutler¹  N. Buapong¹  S. Anuntalabhochai¹ 

关键词: Longan;    hypomethylation;    vernalization;    HAT-RAPD;    temperature dependence;    Spinach;   

DOI  :  10.3844/ajassp.2009.361.367

学科分类：自然科学（综合）

来源: Science Publications

【 摘 要 】

In this study, the HAT-RAPD technique was used to detect DNA methylation in the four plant species, rice (Oryza sativa), petunia (Petunia hybrida), spinach (Spinacea oleracea L.) and longan (Dimocarpus longan Lour.), which were treated using 5-azacytidine, potassium chlorate (KClO₃) and a low temperature induction. Rice and petunia were chosen to be induced because in these species it is known that 5-azacytidine is able to induce hypomethylation in their genomes leading to morphological changes, in particular floral induction, in the developing plants. Using the HAT-RAPD technique, DNA methylation was detected using the restriction enzymes HpaII and MspI in rice and petunia (as expected) and in spinach, but was found to be absent in longan, which suggests that floral induction in longan is undetectable in longan.

【 授权许可】

Unknown